07-06-2020 Council Work Session Packet

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This document is the Intellectual Property of SGS AXYS Analytical Services Ltd. and contains Proprietary and Confidential Business Information. It may not be reproduced or distributed without written permission of the owner. © SGS AXYS Analytical Services Ltd, 2020. SGS AXYS Analytical Services Ltd, 2045 Mills Road W., Sidney, BC, Canada, V8L 5X2. Tel. (250) 655-5800, fax (250) 655-5811. This is not a controlled document; it is subject to change without notification. SGS AXYS Analytical Services Ltd. MSU-110 Rev 14, 28-May-2020 Summary of MLA-110 Rev 02 Ver 07 Page 8 of 23 SGS AXYS confidential, not to be distributed without written permission 4.0 QUANTIFICATION PROCEDURES Calculations Target compounds are quantified using the isotope dilution/internal standard method, comparing the area of the primary (quantifying) transition product ion of the target compound to that of the 13C-labelled or deuterium labeled standard and correcting for response factors. Mean relative response factors (RRF), determined from the multi-level initial calibration series are used to convert raw peak areas in sample chromatograms to final concentrations as follows:              = sample of weight 1 RRF Std Qt of weight Std Qt of area Target of area Target of ionConcentrat where          = Target of weight Std Qt of weight Std Qt of area Target of area RRF and the Qt Std is either the surrogate or the internal standard The isotopically labeled analog of an analyte (surrogate) is used for quantitation (Isotope Dilution Quantitation). If a labeled analog is not commercially available, a surrogate with chemical similarity and close retention time is used for quantitation (internal standard quantitation). Final target concentrations are recovery corrected by this method of quantification. 4.1 Reporting Limits Sample Specific Detection Limits (SDL) are determined by converting the area equivalent to 3.0 times the estimated chromatographic noise height to a concentration in the same manner that target peak responses are converted to final concentrations. The SDL accounts for any effect of matrix on the detection system and for recovery achieved through the analytical work-up. Results are reported to the greater of the SDL or the concentration equivalent to the lowest calibration standard analyzed. www.axysanalytical.com Page 13 of 94