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• <br />• <br />• <br />In the lab, analysis was done to identify macroinvertebrates from each sampling <br />site. Using taxonomic keys, SHEP volunteers identified the Kingdom, Phylum, <br />Class, Order and Family of macroinvertebrate organisms. Once identified, samples <br />were sorted, labeled and scored. <br />4.1.4a Field Sampling Results for Hardwood Creek "Above" Site <br />Historical Field Results for Hardwood Creek `Above' Site <br />Date <br /># <br />Identified <br />Family Biotic <br />Index <br />EPT <br />Number of <br />Families <br />Dominant <br />Family <br />Dominant Family <br />Overall <br />Field Sampling Results: <br />09/08/07 <br />162 <br />7.2 <br />4 <br />24 <br />Hyalellidae <br />41% <br />09/20/08 <br />143 <br />6.3 <br />5 <br />19 <br />Decapoda <br />24% <br />9/12/09 <br />193 <br />6.6 <br />4 <br />18 <br />Chironomidae <br />38% <br />9/11/10 <br />121 <br />6.0 <br />3 <br />18 <br />Hyalellidae <br />30% <br />8/20/11 <br />115 <br />5.0 <br />3 <br />13 <br />Gammaridae <br />43% <br />10/2/12 <br />177 <br />5.0 <br />5 <br />18 <br />Heptageniidae <br />39.5% <br />Cross -Check Results: N/A <br />• Ephemeroptera <br />• Plecoptera <br />▪ Tricoptera <br />• Diptera <br />• Coleoptera <br />• Oligochaeta <br />• Other <br />Hardwood Creek `Above' Data Summary <br />Family Biotic Index (FBI): Our 2012 SHEP field sampling results produced a score of <br />5.0 for the Hardwood Creek `Above' site. This score corresponds to a "Good" rating on <br />the Family Biotic Index stream health chart. This is consistent with 2011 results (5.0), <br />and represents a continued improvement in stream health from 2007 (7.2) to 2012 (5.0). <br />A larger, more diverse sample was identified. However, with more families with high <br />tolerance values, the FBI score remained stable. <br />Number of individuals: A large sample offers more confidence for a more reliable data <br />set. SHEP protocol requires a minimum of 100 individual invertebrates to be picked and <br />identified per sample. 177 invertebrates were identified in this sample. This sample size <br />is adequate. <br />