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• <br />• <br />the Family Biotic Index stream health chart. This is consistent with stream health scores <br />from 2010 (5.1) and 2011 (4.4). In 2012, a larger and more diverse sample was <br />identified, but more families with high tolerance values were present, so the FBI average <br />remained stable. <br />Number of individuals: A large sample offers more confidence for a more reliable data <br />set. SHEP protocol requires a minimum of 100 individual invertebrates to be picked and <br />identified per sample. 210 invertebrates were identified in this sample. This sample size <br />is good. <br />Dominant Family: The dominant family was Gammaridae (scuds). Gammaridae <br />dominated 51.4% of the sample, and has a tolerance value of 4 on a scale of 0 -10 (the <br />lower the tolerance value, the lower their tolerance to pollution). They are closely related <br />to Hyalellidae (note the pollution tolerance difference). The distinction between the two <br />families is the flagellum found on the antennae of Gammaridae. They are important food <br />sources for fish and invertebrate predators. They can be extremely abundant in water <br />bodies without fish and are important in the breakdown of organic matter. (Guide to <br />Aquatic Invertebrates of the Upper Midwest, R.W. Bouchard, Jr). <br />Number of Families: In 2012, 20 families were identified in the sample. This compares to <br />11 families in 2011 and 16 families in 2010. In general, a more diverse sample suggests a <br />healthier stream environment. <br />EFT: Ephemeroptera - Plecoptera - Tricoptera (Mayfly - Stonefly - Caddisfly) are three Orders <br />of invertebrates with low tolerance to pollution. The more of these families in a sample, <br />the better. More than 10 -12 families is considered good. In 2012, 4 EPT families were <br />identified in the sample and made up 17% of the sample. <br />4.1.5 Hardwood Creek Overall Data Summary <br />Interpretation of the Hilsenhoff Biotic Index <br />Sampling Sites <br />2007 <br />2008 <br />2009 <br />2010 <br />2011 <br />2012 <br />Hardwood Creek `Above' <br />7.2 <br />63 <br />6.6 <br />6.0 <br />5.0 <br />5.0 <br />Hardwood Creek `Below' <br />Na <br />Na <br />Na <br />5.1 <br />4.4 <br />4.6 <br />Hardwood Creek continues to show improvement, and initial findings indicate that an <br />abundance of habitat and modest organic pollution levels contribute to good overall <br />stream health in this portion of the watershed. <br />The Family Biotic Index score of 5.3 at the Hardwood Creek `Above' site, and the <br />Family Biotic Index score of 4.6 at the Hardwood Creek `Below' site, indicate a stream <br />health score of "Good ". <br />