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4.4.3 Sampling Methodology Team Leader: Katherine Majkrzak Team Members: Trisha Flaherty, May Hangartner, Ryan Hangartner, Jaime Haueter, Alex Haueter, Cathi Lyman - Onkka, Marilyn Radmer, Susan Young SHEP volunteers used the MPCA's multi - habitat monitoring protocol at each monitoring location. At least 20 jabs were taken from across all major habitat types in the reach. Habitat types include snags and Program staff members performed site visits to assure monitoring was performed according to MPCA guidelines and protocols. Lab analysis identified the taxonomic classification of benthic macroinvertebrate samples from each sampling site. Using taxonomic keys, SHEP volunteers identified the Kingdom, Phylum, Class, Order and Family of macroinvertebrate organisms. Once identified, samples were sorted, labeled and scored. 4.4.4a Field Sampling Results for Locke Lake `Above' Site Historical Field Results for Locke Lake Creek `Above' Site Date # Identifie d Family Biotic Index EPT # of Families Dominant Family Dominant Family % Overall Field Sampling Results: 9/28/06 95 5.0 2 12 Hydropsychidae 58% 9/9/07 103 5.1 3 13 Baetidae 26.2% 10/11//08 163 5.7 4 14 Chironomidae 30% 9/13/09 115 6.1 3 18 Chironomidae 37% 9/12/10 123 5.9 4 13 Chironomidae 43% 9/11/11 362 5.4 3 12 Simuliidae 62% 9/9/12 314 5.2 6 18 Chironomidae 29.3% Cross Check Results: 9/20/08 115 4.9 4 17 Hydropsychidae 33% 9/19/09 107 6.7 4 14 Corixidae 36% • Ephemeraptera • Plecoptera • Tricoptera • Diptera ■ Coleaptera • Oligochaeta • Other • • •