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Apparently the algae in Silver View Pond are still obtaining <br />enough nitrogen, because there is a statistically <br />significant relationship between total nitrogen and and the <br />quantity of algae, as measured by chlorophyll (r =0.88; P< <br />0.01)( Figure 8). Presumably, at higher algal densities <br />algae are running short of nitrogen at which time blue - <br />greens begin to obtain nitrogen directly from the air <br />through the process of nitrogen fixation. On two sampling <br />dates, dissolved nitrogen compounds (nitrate plus nitrite) <br />were measured, and five of the six samples were below the <br />detection limit of 50 ug/L ( see Table 2), indicating that <br />the algae were taking up most of the available nitrogen. <br />Algae <br />Given that the system seems to be slightly nitrogen -limited, <br />one would expect blue-green algae to dominate the <br />phytoplankton of Silver View Pond. However, on every <br />sampling date, the algae population of the pond was <br />dominated by a non -blue-green species, Ce_ratium (Table 3). <br />The balance of the arae on each date were either blue - <br />greens or green algae. However, except for :Anabaena, the <br />blue -greens observed in were of a type that can not obtain <br />nitrogen from the air. These results apply to water samples <br />obtained from the middle of each arm of Silver View Pond. <br />Table 3. Estimated composition (by biomass) of <br />phytoplankton in Silver View Pond, 1989. <br />Date % N -Fixing BGa <br />% other BGb % Ceratium % Green <br />6/24 5 10 80 5 <br />7/22 0 30 60 10 <br />8/13 0 0 75 25 <br />9/2 0 50 50 0 <br />a The only nitrogen -fixing blue-green algae (N -fixing BG) <br />observed was Anabaena. <br />b Non -nitrogen -fixing' blue-green algae (non -ti -fixing BG) <br />observed were Oscillatoria or Lynob a and `licrocystis. <br />In contrast, colonies of blue-green algae were observed <br />floating around the margins of the pond on both June 24 and <br />September 2. On June 24 large colonies of Oscillatoria or <br />L.yri bLa (both non nitrogen -fixing blue -greens) and <br />filamentous green algae were observed floating at the <br />surface. On September 2 numerous (at least 50) colonies of <br />the blue-green alga Nostoc had been blown to the shore of <br />the western arm. Nost_oc colonies are hard, hollow green <br />spheres slightly smaller than a ping-pong ball. When <br />examined under a microscope, the colonies were rich with <br />heterocysts, which are the special cells that fix nitrogen. <br />86 <br />